Urea

This method is used for the determination of Urea in drinking, surface and saline waters, omestic and industrial wastes, plants and soils and is applicable to many ranges the most common being 0.05 – 5 µM.

Summary

A red color is formed when urea is heated with 2,3-butanedione monoxime and thiosemicarbazide under acidic conditions. The change in absorbance is measured at 520 nm.

Limitations

Turbid samples must be filtered or centrifuged prior to determination. Samples with background absorbance at the analytical wavelength may interfere.

Availability